Notes on fermenter feed supplement
1. Aseptic operation: Throughout the feed supplement, aseptic operation rules must be strictly adhered to in order to prevent microbial contamination.
2. Precise control: no matter which way of feed supplement, the amount of supplements should be precisely controlled to ensure the stability of the fermentation process.
3. Equipment maintenance: Regular inspection and maintenance of the equipment to ensure its normal operation.
4. Recording and analysis: All data during the feeding process should be recorded in detail and analyzed to optimize the feeding strategy.
2. Precise control: no matter which way of feed supplement, the amount of supplements should be precisely controlled to ensure the stability of the fermentation process.
3. Equipment maintenance: Regular inspection and maintenance of the equipment to ensure its normal operation.
4. Recording and analysis: All data during the feeding process should be recorded in detail and analyzed to optimize the feeding strategy.
Five ways to feed supplement of stainless steel fermenter
1. Pin-type feed supplement: material is injected directly into the fermenter through flame-sterilized pins. The operation is simple, but the sterility of the operation cannot be guaranteed.
2. Push-valve feed supplement: Optimized for Pin-type, it avoids re-exposing the inside of the sterilized refill bottle to the air. Easy to operate, but all operations must be completed before the vessel is sterilized.
3. Four-valve group feed supplement: feed valve, exhaust valve, sampling valve and return valve are used in combination to control the flow direction and speed of the refill material, effectively managing the material flow and pressure balance in the fermenter. Operation and maintenance are relatively complex, and the maintenance cost is also higher.
4. Flowmeter feed supplement: Use flowmeter to measure the flow of materials, and adjust the supplement amount by controlling the valve or pump.
5. Metering cup feed supplement: The supplement amount is determined by the volume of the metering cup, and then the material is delivered to the fermenter through the control valve or pump. The supplement accuracy is better than the flow meter solution, but the risk of bacterial contamination is higher.
2. Push-valve feed supplement: Optimized for Pin-type, it avoids re-exposing the inside of the sterilized refill bottle to the air. Easy to operate, but all operations must be completed before the vessel is sterilized.
3. Four-valve group feed supplement: feed valve, exhaust valve, sampling valve and return valve are used in combination to control the flow direction and speed of the refill material, effectively managing the material flow and pressure balance in the fermenter. Operation and maintenance are relatively complex, and the maintenance cost is also higher.
4. Flowmeter feed supplement: Use flowmeter to measure the flow of materials, and adjust the supplement amount by controlling the valve or pump.
5. Metering cup feed supplement: The supplement amount is determined by the volume of the metering cup, and then the material is delivered to the fermenter through the control valve or pump. The supplement accuracy is better than the flow meter solution, but the risk of bacterial contamination is higher.
Feed supplement of glass fermenter
1. If conditions permit, we recommend that the glass fermenter and the bottle be put into the sterilizer for autoclaving, and the silicone tube connecting the glass fermenter and the bottle should be tightly clamped with hose clamps to effectively prevent microbial contamination.
2. If the conditions are not permitted, such as ammonia can not be sterilized, fermentation culture medium and supplement medium sterilization conditions are not consistent, the capacity of the sterilizer can not be met, and so on. If it is necessary to sterilize glass jars and bottles separately, then it is necessary to connect luer check valves at both ends of the connection to avoid bacteria contamination due to the connection pipes.
Here is the Holves brand website, https://www.bjholves.com/. Providing different types of industry information, technical knowledge, and solutions, we have developed and produced several new laboratory fermenter, bioreactor, tangential flow filtration system and other equipment to meet your needs from experimental to industrial production.
2. If the conditions are not permitted, such as ammonia can not be sterilized, fermentation culture medium and supplement medium sterilization conditions are not consistent, the capacity of the sterilizer can not be met, and so on. If it is necessary to sterilize glass jars and bottles separately, then it is necessary to connect luer check valves at both ends of the connection to avoid bacteria contamination due to the connection pipes.
Here is the Holves brand website, https://www.bjholves.com/. Providing different types of industry information, technical knowledge, and solutions, we have developed and produced several new laboratory fermenter, bioreactor, tangential flow filtration system and other equipment to meet your needs from experimental to industrial production.
